Hello, There are several method of statistical analysis between groups such as ANOSIM, ANOVA, PERMANOSIM PERMANOVA. This Posting is about Analysis of Similiarity (ANOSIM). The interpretation of ANOSIM is well explained below of Google WIKIPedia. https://en.wikipedia.org/wiki/Analysis_of_similarities Theory, equation, interpretation, etc. Here is about how to statistically analysis the data by R ..