Hello, This posting is about 16S rRNA microbiomal function prediction analysis. There are three main tools for functional prediction of bacterial microbiomes such as PICRUSt, FAPROTAX, Tax4Fun2. All of these contains weak points and strong points. In my case, i prefer to use Tax4Fun2 because of analysis pipeline. Brifely, 16S rRNA sequence data will be blasted, and highest similarity chromosome ..